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aar 016  (Alomone Labs)


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    Structured Review

    Alomone Labs aar 016
    Aar 016, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/aar+016/Anti-%CE%B22-Adrenergic+Receptor+Antibody/pmc12648565-59-146-147
    Average 93 stars, based on 8 article reviews
    aar 016 - by Bioz Stars, 2026-09
    93/100 stars

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    Incubation:

    Article Title: Promoting differentiation of human-induced pluripotent stem cells into sinoatrial node-like cells through programmed regulation of AMPK signalling pathway
    Article Snippet: .. Cells were seeded in a μ-Slide 8 Well (80827, ibidi, Germany) coated with Matrigel at a density of 2 × 10 4 per well for 48 h. Cells were fixed with 4% (w/v) paraformaldehyde (PFA) for 15 min at room temperature, permeabilized with 0.2% Triton X-100 for 15 min at room temperature, blocked with 5% (w/v) BSA in PBS for 30 min, and then incubated with the following primary antibodies: anti-SHOX2 antibody (1:200) (ab55740, Abcam, USA), anti-HCN4 antibody (1:100) (MA3-903, Invitrogen, USA), anti-NKX2.5 antibody (1:400) (ab91196, Abcam, USA), anti-cardiac Troponin T antibody (1:200) (ab55740, Abcam, USA), anti-cardiac Troponin T monoclonal antibody (1:200) (MA5-12960, Invitrogen, USA), anti-alpha 1a adrenergic receptor/ADRA1A antibody (1:50) (ab137123, Abcam, USA), anti-muscarinic acetylcholine receptor 2/CM2 antibody (1:100) [(31-1D1), Abcam, USA), anti-alpha 2a adrenergic receptor/ADRA2A antibody (1:50) (bs-1062R, BIOSS, China), anti-β1-adrenergic receptor (extracellular) antibody (1:200) (AAR-023, Alomone, Palestine), and anti-β2-adrenergic receptor (extracellular) antibody (1:50) (AAR-016, Alomone, Palestine), overnight at 4°C according to the supplier’s instructions. .. Plates were then washed three times with PBS for 5 min each on a destaining shaker and incubated with species-specific fluorescently conjugated secondary antibodies: Alexa Fluor 488-labelled goat anti-rabbit IgG (A11008, Invitrogen, USA) and Alexa Fluor 488-labelled goat anti-mouse IgG (A-11001, Invitrogen, USA) for 1 h at room temperature.



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